Isolation and Expression Analysis of Meristem Gene <i>CLV</i>2 and Carpel Generation Gene (<i>CRC</i>) from Multi-Carpel Structure of <i>Thermopsis turcica</i>

Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces flower pistils with unusual morphological properties. aim this study was to determine the factors controlling interesting feature plant. Particularly features meristem and that mutations two genes helps occurring number organs can cause abnormalities, This has attempted explore turcica, young bud Arabidopsis CLV2, CRC gene homologs were isolated analyzed different tissues. First, total RNA from first thread degenerated primers synthesized cDNA expressions, second a partial length cloning process made replicated set analyses. full-length related RACE analysis carried out sequencing process. real-time PCR method applied for functional analyses buds, sepals, petals stamen relative expression tissue pistils. Full-length sequence CLV2 2557 bp, encoded protein 716 amino acids, 901 gene—bp, 174 identified as acids. Alignment phylogenetic found be similar other types legumes homologous sets. Real-time (RT-qPCR) results courses genes.